Expression profile of genes encoding for Heat Shock protein of Aspergillus flavus during interaction with maize

Abstract

Maize is defenceless against sullying in association with the destructive infectious agent Aspergillus flavus. Illness follows the decline in the level of grains and contamination of a little part, alongside an extraordinarily malignant growth originating the formation of harmful mycotoxin known as aflatoxin. The contamination caused by aflatoxin in maize or similar host originated by Aspergillus flavus tends to an outrageous money related mishap and threat human prosperity. Thusly, this examination was finished to appreciate the establishment of the colony of Aspergillus flavus on maize kernel. Seeds of maize were taken for the following project work. Aspergillus flavus strain BT05 was spoiled with the parts of maize (i.e., seeds) under different time conditions and autoclaved treatments and after the infection of A. flavus on the maize parcel, RNA was extracted to examine the value of verbalization of the given fungal strain i.e., A. flavus similarly as the aflatoxin quality of being dependable will be inspected. To fathom the instrument of A. flavus related with maize parcel, quality articulation investigation was finished by using RT-PCR for picked characteristics of two heat shock protein quality, HSP70, HSP90 and aflatoxin reliable ability will definitely be in frail in maize. Our results provide cover as a foundation for clear understanding of the generation of aflatoxin in maize and also provide a new snippet of the data to administer aflatoxin contamination. And at last it was observed that the gene expression of both the genes HSP70 and HSP90 are higher on the third day of the fungal infection but on the 5th day of the fungal infection, decrement in the gene expression of both the genes was observed. On both observed days of fungal infection gene expression of HSP90 was much higher than HSP70, also the decrement rate of gene expression of HSP70 was higher than HSP90