Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9116
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKumar, Pawan-
dc.contributor.authorSaini, Mahak-
dc.contributor.authorSaini, Mahak-
dc.contributor.authorWarghat, Ashish R.-
dc.contributor.authorMalhotra, Nikhil-
dc.contributor.authorSood, Archit-
dc.date.accessioned2023-01-11T11:13:33Z-
dc.date.available2023-01-11T11:13:33Z-
dc.date.issued2014-
dc.identifier.urihttp://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9116-
dc.description.abstractCell suspension cultures of Arnebia euchroma were established from the friable callus on liquid Murashige and Skoog medium supplemented with 6-benzylaminopurine (10.0 μM) and indole-3-butyric acid (5.0 μM). Salicylic acid was used to study its effect on the enzymes which participate in shikonin biosynthesis with respect to metabolite (shikonin) content in the cell suspension culture of A. euchroma. In our study, phenylalanine ammonia lyase and PHB geranyltransferase were selected from the entire biosynthetic pathway. Results showed that phenylalanine ammonia lyase is responsible for growth and PHB geranyltransferase for metabolite production. Salicylic acid exhibited an inverse relationship with the metabolite content (shikonin); salicylic acid (100 μM) completely inhibited shikonin biosynthesis. The results presented in the current study can be successfully employed for the metabolic engineering of its biosynthetic pathway for the enhancement of shikonin, which will not only help in meeting its industrial demand but also lead to the conservation of species in its natural habitat.en_US
dc.language.isoenen_US
dc.publisherJaypee University of Information Technology, Solan, H.P.en_US
dc.subjectArnebia euchromaen_US
dc.subjectBoraginaceaeen_US
dc.subjectCell suspension cultureen_US
dc.titleEffect of Salicylic Acid on the Activity of PAL and PHB Geranyltransferase and Shikonin Derivatives Production in Cell Suspension Cultures of Arnebia euchroma (Royle) Johnst—a Medicinally Important Plant Speciesen_US
dc.typeArticleen_US
Appears in Collections:Journal Articles

Files in This Item:
File Description SizeFormat 
s12010-014-0838-x (1).pdf604.52 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.