Prevalence and Characterization of Cronobacter spp. from Various Foods, Medicinal Plants, and Environmental Samples

Abstract

Dairy or non-dairy based products were explored to determine the prevalence, molecular characterization, and antibiotic susceptibility of Cronobacter spp.The isolationwas done as per ISO 22964:2006 on chromogenic media followed by further confirmation by biochemical- and 16S rRNA-based identification. From219 samples, the chromogenic agar assay and biochemical tests yielded presumptive 45 isolates.Among them, only 36 isolates showed 282 bp band amplified from ITS-G gene confirming as Cronobacter sakazakii. The Cronobacter spp. prevalence was highest in herbs and spices (34 %) while environmental samples had contamination rates of 23 % indicating plants as a possible reservoir of this pathogen. All the isolates were resistant to b-lactam derivatives (68 %), macrolides (88.6 %), and aminoglycosides (79.9 %) but susceptible to phenicoles (31.6 %) and tetracyclines (15 %)derivatives.The resultsemphasize the screening of plant materials before their incorporation in food matrices.