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http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9028| Title: | InsilicoAnalysisRevealedHigh-riskSingleNucleotidePolymorphismsinHumanPentraxin-3GeneandtheirImpactonInnateImmuneResponseagainstMicrobialPathogens |
| Authors: | Thakur, Raman Shankar, Jata |
| Keywords: | PTX-3 In silico Aspergillusfumigatus lipopolysaccharide |
| Issue Date: | 2016 |
| Publisher: | Jaypee University of Information Technology, Solan, H.P. |
| Abstract: | Pentraxin-3(PTX-3)proteinisanevolutionaryconservedproteinthatactsasasolublepattern-recognitionreceptorforpathogensandplaysimportantroleininnateimmuneresponse.Itrecognizesvariouspathogensbyinteractingwithextracellularmoietiessuchasglactomannanofconidia(Aspergillusfumigatus),lipopolysaccharideofPseudomonasaeruginosa,StreptococcuspneumoniaandSalmonellatyphimurium.Thus,PTX-3proteinhelpstoclearthesepathogensbyactivatingdownstreaminnateimmuneprocess.Inthisstudy,computationalmethodswereusedtoanalyzevariousnon-synonymoussinglenucleotidepolymorphisms(nsSNPs)inPTX-3gene.ThreedifferentdatabaseswereusedtoretrieveSNPdatasetsfollowedbysevendifferentinsilicoalgorithmstoscreennsSNPsinPTX-3gene.SequencehomologybasedapproachwasusedtoidentifynsSNPs.ConservationprofileofPTX-3proteinaminoacidresidueswerepredictedbyConSurfwebserver.Intotal,10high-risknsSNPswereidentifiedinpentraxin-domainofPTX-3gene.Outofthese10high-risknsSNPs,4werepresentintheconservedstructuralandfunctionalresiduesofthepentraxin-domain,hence,selectedforstructuralanalyses.Theresultsshowedalterationintheputativestructureofpentraxin-domain.Predictionofprotein–proteininteractionsanalysisshowedassociationofPTX-3proteinwithC1qcomponentofcomplementpathway.DifferentfunctionalandstructuralresiduesalongwithvariousputativephosphorylationsitesandevolutionaryrelationshipwerealsopredicteforPTX-3protein.ThisisthefirstextensivecomputationalanalysesofpentraxinproteinfamilywithnsSNPsandwillserveasavaluableresourceforfuturepopulatiobasedstudies. |
| URI: | http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9028 |
| Appears in Collections: | Journal Articles |
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