Study on Beta Sitosterol Biosynthetic Pathways and it’s Potential Applications

Abstract

Sterols are strong steroid liquors that are found in the cell layers of animals, plants, and microorganisms. They are classified as zoosterols, phytosterols, and mycosterols. Beta-sitosterol is the only phytosterol that has the same structure and function as cholesterol. Because of its structural similarity to cholesterol, Beta-sitosterol is highly utilized in a vegan diet. With these unique properties, beta sitosterol is now widely employed in a variety of pharmaceuticals for the treatment of cancer, cardiovascular disease, and other disorders. In this thesis, I discuss the many positive aspects of the chemical betasitosterol, as well as its impact on human health and pharmacological therapy. I've also discussed the approach for analyzing and quantifying beta sitosterol levels using various methods such as HPLC and quick purification procedures. Aside from that, I've stated the yeast Saccharomyces cerevisae engineering ways by which we can conduct the manufacture of this chemical Beta Sitosterol by alterations in enzymes and genes in the Mevalonic Acid Pathway.