Synergistic Studies of Peptide, Plant Extract against Gram Positive and Gram Negative Bacteria

Abstract

There is an alarming increase in antimicrobial resistance worldwide and India is also facing this similar problem. The high accessibility of antibiotics over the country has majorly contributed in the high antimicrobial resistance observed. Although therapeutic industries have produced a number of new antibiotics in the last few decades, still bacteria manage to attain resistance against these drugs. Such facts are cause for distress, because due to new multi- resistant microorganisms number of patients in hospitals have repressed immunity. Synergistic studies are one of the approach used to deal with antimicrobial resistance. So in this we have used combination of peptide with antibiotics and antibiotics with various plant extracts for their antimicrobial synergy against gram negative and gram positive bacteria. In this project we have used different antibiotics (gentamycin, vancomycin and ampicillin) and synthetic peptide (O3S) along with combination of different plant extracts (Cestrum nocturnum and Witharnia somnifera) to check whether they show synergistic activity against microorganism. As in synergistic response, the combination of antibiotics work together to produce more effective response than the each antibiotic produce individually.