Optimization of Tannase Production from Aspergillus SP

Abstract

Tannin acyl hydrolase (EC 3.1.1.20) which is commonly referred as tannase is one of the hydrolytic microbial enzymes. Tannase is an industrially important enzyme and has several applications in various industries such as foods, animal feeds, cosmetics, pharmaceutical, chemical, leather industries etc. Realizing the importance of the enzyme tannase, the present study was aimed to establish a solid state fermentation in order to obtain the enzyme and further the isolated enzymes’s physicochemical properties are observed through RSM (Response Surface Methodology) and Tannase assay. Based on the RSM approach, the maximum tannase activity was observed i.e 4.86 U/ml thereby depicting that the most ambient condition of production are pH 4.0, temperature 30̊C, incubation period of 6 days and moisture level of 10ml in 3g of substrate.