Arginase isolation from Bacillus Megaterium and characterization

Abstract

Arginase is a widely known enzyme of the urea cycle that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. The action of arginase goes beyond the boundaries of hepatic ureogenic function, being widespread through most tissues. B.megaterium is an rod shaped , a natural PHB producer, gram positive aerobic spore forming bacteria usually found in the widely diverse habitats and its among biggest bacteria.in this experiment we used bacillus megaterium and inoculated it into nb broth and used that growth to see the growth of the bacteria and then afterwards being used for the staining process in which we will come to see the gram positive bacteria after the staining in which the stain usually binds with the bacteria then either the bacteria will stay purple or then turn to pink if then it turn to pink then it’s a gram positive which will show some rod shaped like mainly in chains and concluded that we able to see the proper growth of the bacillus megaterium ,it's been also called as a big beast.