Tyrosinase gene polymorphism in genetic susceptibility towards Vitiligo

Abstract

Vitiligo, a common depigmentation disorder, caused by loss of melanocyte from the lesion site. Variousaspects are thought to play a role in inducing Vitiligo. Up regulated expression of tyrosinase in the melanocytes from perilisonal skin as compared to normal skin of vitiligo patient. Genetic variations in genes may contribute to disease pathogenesis. In this study, we investigated the association of tyrosinase gene polymorphism rs6336and rs61753185 with vitiligo susceptibility among north India population. For the study we recruited 103 patients with vitiligo and 92 matched control samples without any history of vitiligo or any other autoimmune disorder .Informed consent from all the patients were collected. We showed that the two variants in TYR gene do not influence the susceptibility to vitiligo in an individual. We took help of many techniques which made our work convenient. Through the advantages of polymerase chain reaction- restriction fragment length technique, genotypes were effectivelyattained for the cases and controls. Accordingly, appropriate statistical approaches were applied to explain the relation of variants with the susceptibility of the allelic frequencies of these SNPs between cases and control were, rs6336: p=0.0236; rs61753185: p=0.5787, which suggested no association of minor allele of both the SNP with the disease. The results showed that the SNP rs6336 may have role in protection against disease while, the SNP rs61753185 did not show any association with vitiligo susceptibility