Expression Analysis of Transcriptional Factor aflR during Aspergillus Flavus-maize Kernel Interaction

Abstract

Maize is vulnerable to contamination with the harmful pathogen Aspergillus flavus. Disease results in the decrease in the standard of grains and pollution of bits along with an exceptionally cancer-causing mycotoxin aflatoxin. The pollution of aflatoxin in maize or maize related items blended by Aspergillus flavus makes extreme financial misfortune and danger human well-being. Along these lines, this investigation was complete to comprehend the colonization of A. flavus on maize bits. Maize seed was taken for work. A. flavus (BT05) refine was tainted with maize part under various condition and time span and after the colonization of A. flavus on maize portion the RNA was separated for investigation of quality articulation of A. flavus just as the aflatoxin dependable quality will be examined. To comprehend the instrument of A. flavus associated with maize portion, gene expression analysis was done by utilizing RT-PCR for chose qualities of two aflatoxin pathway gene i.e., aflR and aflD and aflatoxin dependable quality will be in powerless in maize part. Our outcome fills in as an establishment for understanding the aflatoxin generation in maize-part and offer new piece of information to oversee aflatoxin defilement in yields. RT-PCR was done with the two aflatoxin gene and look upon the regulation of the gene on maize under various condition. And finally it was observed that in 3rd day of treatment it represents the high gene expression of gene aflR. Where as in aflD there was low gene expression. After 5th day of treatment there was low expression of the aflD genes but it aflR expression was high. And finally we concluded that in 3rd day and 5th day the expression of aflR gene is high and the expression of aflD gene is low.