Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/6864
Title: Production of Somatic Seeds of Nothapodytes Nimmoniana and Testing the Pharmacological activity of the Plant extract
Authors: Rajta, Ankit
Sood, Hemant [Guided by]
Keywords: Morphological analysis
Somatic seeds
Nothapodytes nimmoniana
Issue Date: 2017
Publisher: Jaypee University of Information Technology, Solan, H.P.
Abstract: The present study was conducted to optimize the culture conditions for the production and germination of artificial seeds through somatic embryogenesis in Nothapodytes nimmoniana. The tree belongs to family Icacinaceae. The plant is known for important secondary metabolite camptothecin, an important anti-cancer alkaloid. The tree is commonly found in the Western Ghats of Maharashtra (India). Due to overexploitation and lack of organized cultivation, the wild populations of the tree have declined fast. In addition, there are a number of other constraints mentioned in the literature for the propagation and conservation of the tree through seed propagation. Low seed germination rate and the immense exploitation of the tree for obtaining the high market value anti-cancer alkaloid, camptothecin has resulted in the endangered status of the species. Plant tissue culture offers various effective measures for meeting the demand of camptothecin and rapid clonal propagation for tree conservation. Hence, artificial seed technology can be used for the large-scale production of plantlets which could later be used for the production of camptothecin. We have carried out a comparative analysis of plant extract prepared from tissue cultured plantlets and field grown shoot and roots on breast cancer cell lines to predict the efficacy of its anticancerous effects. Hydroalcoholic extraction of the samples and their quantification through HPLC has been done which result in (0.03%) camptothecin in in-vitro plantlets, field grown leaves (0.87%) and field grown stem (0.17%). In cell line study, we achieved high efficacy of field grown extract on cancer cell death as compared to in-vitro grown plantlets. Difference in camptothecin content quantified through HPLC can be attributed to stress conditions that are faced by field grown plants which contributes to secondary metabolite production as compared to the sustained environment provided to in-vitro plants, difference in age group of fields grown plants (5 – 6 years) and in-vitro plantlets (2 – 3 months) old, origin of explants used for culturing in-vitro plants and use of different growth hormones for culturing the in-vitro plants etc. Cytotoxic studies have been performed on HEK 293(Human Embryonic Kidney) to check the effect of plant extract on normal cells. IC50 value obtained for all the samples was quiet high which indicates that camptothecin is safe for normal cells similarly anticancerous effects of Nothapodytes nimmoniana crude extract have been assessed on MCF-7 and MDA-MB viii 231 where MDA-MB have shown more sensitivity toward the extract as compared to MCF-7 cells.
Description: Dual Degree Project Report
URI: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/6864
Appears in Collections:B.Tech. Project Reports



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.