Exploring biofilm forming capacity of A.baumannii from different clinical sources and screening copper nanoparticles for antibiofilm activity.

Abstract

A total of 48 A. baumannii strains were collected from Department of Microbiology (Bacteriology division), AIIMS, New Delhi during the study period. Clinical source of isolation of A. baumannii included patient’s pus, respiratory and peritoneal fluid etc. Collected strains were characterized by standard biochemical assays (carbohydrate and amino acid utilization). A. baumannii (ATCC 19606) which is a type strain was also made in use as control against antimicrobial susceptibility analysis and biofilm production assay. Antimicrobial susceptibility test (AST) and minimum inhibitory concentration (MIC) of different antibiotics against isolates of A. baumannii were performed according to CLSI guidelines 2015 and ICMR standard operating procedure, bacteriology 2015. AST and MIC were done using Kirby Bauer’s disk diffusion method and E- strips test, respectively. Motility assays were performed which includes swarming and twitching to observe that if the pathogen is motile or not and to what extent motility is helping in dispersal of the pathogen in new environment. Antibiofilm activity of copper nanoparticles synthesized by green synthesis, amalgam with catharanthus-rosesus etc. was screened by antibiofilm assay.