Development of Antigen-antibody Interaction Based Cost Effective Teaching Kit for in-house Application

Abstract

Antigen antibody based teaching kits are majorly used in research and educational activities, apart from its highly standardized and customized version finding diagnostic application too. To achieve the goal to develop a cost effective In-house teaching kit, we utilized M. fortuitum culture as a source of antigen in the form of cellular protein lysate and serum from mice model infected with wild type M. fortuitum as a source of antigen which is freely available in our laboratory, as it was extracted in earlier studies conducted. We were able to effectively standardize the protocol for extraction, the use of a suitable buffer combination for interaction of antigen with antibody in a precipitation reaction. We were able to demonstrate the optimum amount of antigen concentration required for carrying out quantitative precipitation and gel based Immuno Diffusion Assay. For future aspects research can be focused on standardizing and refining gel-based antibody-antigen interaction method for utility as inhouse teaching kit.