Comparative Genome Analysis of Diarrheagenic and Non-pathogenic Strains of Escherichia Coli

Abstract

Diarrhea is the most important and common cause of deaths in young children in India. It is the disturbance in the normal mechanism of secretion and absorption of water resulting in excess loss of water in faeces. It is the state of having minimum of three loose or liquid bowel movements each day and most commonly it can last for few days. It is responsible for causing dehydration because of fluid loss. Diarrheagenic Escherichia coli (DEC) is one of foremost causative agent of diarrhea. It is considered to be the most common source of epidemic and endemic diarrhea universally. Although the majority of diarrheal illness is resolved itself without any treatment and dehydration can typically be managed by oral rehydration therapy. In severe cases that have a greater risk of causing deaths, it is necessary to prevent diarrhea. In order to achieve this aim, present study has been carried out which focuses on rapid identification of the pathogen. Genomes were compared and two unique proteins clpV1 family T6SS ATPase and type six secretion system protein were discovered to be present only in the pathogenic strains of E. coli. With the help of these proteins we can clearly differentiate between the pathogenic and non-pathogenic strains of E. coli and can directly identify and detect the pathogen in the stool samples