Optimization of L Arginase Production from Lactobacillus Species

Abstract

Arginases are anti-cancerous enzymes which can be used in the treatment of debilitating human cancers by nutritional starvation therapy. The antitumor activity of these enzymes is found to be effective in countering wide range of cancers. One Variable at a time (OVAT) technique was employed to optimize the production of arginase from Lactobacillus acidophilus. Various physical parameters such as volume, inoculum age, inoculum size and incubation time were studied for their effect on the production of arginase and so on its activity and hence were optimized. The location of the enzyme was intracellular and the optimal values of physical parameters for arginase production were: volume = 200ml, inoculum age = 10 hours, inoculum size = 1% and incubation time = 24 hours.