Computational Tools and Resources for CRISPR/Cas 9 Genome Editing Method

Abstract

CRISPR/Cas9, the popular genome editing method, has wide applications ranging from genetic engineering of plants, modifying bacterial genomes for production of therapeutic, agricultural, industrial bioproducts. It would be an understatement to claim that it has indeed revolutionized the field of gene and genome editing. To aid the researchers in designing the optimal sequences for CRISPR/Cas9 experiments, a multitude of bioinformatics tools and resources have been developed and are currently used. This review aims to provide an up to date list of tools, web servers, databases, and other resources that can be used for editing the genome. We hope that such a compilation will enable researchers to select the resources based on functionality, ease of use, availability, and specific research needs.