Formulation and Evaluation of Dual Drug Loaded Nano Lipid Carrier Based System

Abstract

Background: Ciprofloxacin and Clotrimazole are used as antimicrobials. There are various diseases in which mechanism of disease causing microbe is unknown; therefore a formulation which acts by more than one mechanism of action might prove beneficial in the disease.Since both drugs have different mechanisms of actions. Thus attempts were made, to prepare a dual drug loaded formulation. Objective: The aim of the research work is to prepare and evaluate dual drug loaded NLCs based formulation which has advantages of both drugs i.e. Ciprofloxacin and Clotrimazole. Method:NLCs of dual drugs were prepared by emulsification-ultrasonicationmethod using stearic acid as lipid. Results and discussion: The in-vitro studies were carried out at phosphate buffer pH 7.4 at 37±2º C. NLCs in all formulations were found to be spherical in shape; with size ranging from 200-800 nm. All formulations exhibited an in-vitro release of 65-85 % in 24hour. % Drug loading of (F4) was found to be highest. Therefore it was selected as best formulation. Conclusion: The present study was carried out to develop the NLCs of dual drugs (ciprofloxacin and clotrimazole) using stearic acid. Thus to conclude dual drug loaded NLCs may provide advantages of both drugs.