Development of Gene Tagged SNP Markers for Functional Analysis of Starch Branching Enzyme Genes in Oryza Sativa Indica SubSP

Abstract

The starch branching enzyme genes and other genes like starch synthase genes, granule bound starch synthase gene play a crucial role in starch biosynthesis. These genes are involved in various physicochemical properties like amylose content and gelatinization temperature which are very important in determining the rice quality. The functional properties of these genes in 11 Indian rice genotypes were analysed. We focused on the Indica varieties which are consumed more in India and their starch properties affect the insulin levels of diabetic consumers. We tried to develop the SNPs to study variation in SBE gene sequences. The rice seeds were germinated at optimum temperature and genomic DNA was isolated from its seedlings. Amplification of SBE gene 1and 3 was carried on various genotypes. The physicochemical studies were also done to relate the glycemic levels and resistant starch with rice properties. In this study, we also estimated the amylose content of 11 rice varieties which showed that s5 variety (RR seed) has intermediate amylose levels which is preferable for consumption and grain elongation test was also performed to study the rice eating and cooking quality. Rice consumption has a significant effect on diabetic population so discovering such varieties with optimum levels of glycemic index is required which can be achieved by studying the genes involved in starch biosynthesis. Such candidate genes can be utilized for planning genetic manipulation studies to produce desirable rice varieties.