Expression Analysis of Key Genes in Shoot Cultures of Swertia Chirayita Fed With Different Precursors in Vitro

Abstract

Swertia chirayita is a high value medicinal herb having therapeutic properties. The medicinal properties conferred by this herb are associated with the presence of a well-known range of secondary metabolites with pharmacological significance. These secondary metabolites include, swertiamarine,amarogentin and mangiferin. Different analysis on these compounds related key genes is already done, but there is lack of study on corresponding product which is get enhanced with the intermediate compounds or some other end product. Different key genes which were taken in such a way for them the gene expression studies is done which give significant association with the production of these intermediate and final produced secondary metabolite. So this study identified some key genes which are having potential importance in the molecular level and in metabolic engineering of S.chirayita. These key genes which are used for enhancement of the secondary metabolite production and from here production of these secondary metabolite get increased