Analogy of ISSR and RAPD Markers for Comparative Analysis of Genetic Diversity among different Jatropha curcas Genotypes

Abstract

The phylogenetic relationships of 13 Jatropha genotypes from different parts of the India were analysed using 34 polymerase chain reaction (PCR) markers (20 random amplified polymorphic DNAs (RAPDs) and 14 inter simple sequence repeats (ISSRs)). Amplification of genomic DNA of the 13 genotypes, using RAPD analysis, yielded 107 fragments that could be scored, of which 91 were polymorphic, with an average of 4.55 polymorphic fragments per primer. Number of amplified fragments ranged from one (OPA20, OPB19, OPD13) to nine (OPA18) and which varied in size from 200 to 2,500 bp. Percentage of polymorphism ranged from 40% (OPB18) to a maximum of 100% (14 primers). Resolution power ranged from a minimum of 0.153 (OPA20, OPB19) to a maximum of 11.23 (OPB15).