Synthesis of copper nanoparticles via green synthesis and analyzing potential effect of copper nanoparticles on Drosophila melanogaster

Abstract

Copper nanoparticles have became center of attraction in last decades. With their broad range of application in pharmaceuticals, medicine and drugs, we can’t neglect the effect copper nanoparticles on human and environment. Their way to biosphere should be highly regulated and tested. The established physico-chemical methods came with lots of limitations and disadvantages when it comes to their usage in synthesizing particles that can be used in biological or pharmaceutical applications. Thus, we need a method which can be sustainable, safe and eco-friendly. This paved the way for term Green nanobiotechnology, where biological entities are used in producing nanoparticles. Our study aims to synthesize copper nanoparticles using extract of plant Myrica esculenta and also test the effect of copper nanoparticles on widely used animal model, Drosophila melanogaster. We were able to synthesis copper nanoparticles by using equal amount of copper sulfate pentahydrate and plant extract. Other factor affecting the synthesis of copper nanoparticles like pH, incubation time period and temperature were also studied. Also we tested the copper nanoparticles in three different concentration on Drosophila, where we found significant result in control versus the treated media of flies.