Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/5095
Title: Isolation and characterization of Carbonic Anhydrase producing bacterial isolates for CO₂ conversion
Authors: Bhatti, Ishika
Nadda, Ashok Kumar [Guided by]
Keywords: Carbonic anhydrase
Calcium carbonate
Sequestration
Issue Date: 2022
Publisher: Jaypee University of Information Technology, Solan, H.P.
Abstract: Rapid industrialization and burning of fossil fuels is one of the major causes of global warning which has led to the deterioration of a healthy environment. Microbial carbonic anhydrase has been employed for the reduction of CO2 level in the environment. Soil sample was used in this study in order to search for CA-producing bacteria. In the bacterial cultures, eight isolates were chosen after screening for CA activity. It was found that Isolate no. 5 had the highest concentration of CA activity (1.58 U/ml) out of all the bacteria tested. Several production parameters were optimized for CA production. Incubation temperature 20°C, media nutrient broth, and inoculum volume 0.25%v/v, pH 7 was optimized. The crude enzyme was examined for the conversion of CO2 into calcium carbonate (CaCO3). Crude CA was shown to have a CO2 conversion. Bacterial CA precipitation of calcite can be exploited for the sequestration of carbon, can be used in a variety of applications such as paper industry, cements, and construction materials.
URI: http://ir.juit.ac.in:8080/jspui//xmlui/handle/123456789/5095
Appears in Collections:Dissertations (M.Sc.)



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