Sequence and Structure Based Analysis of K-RAS Gene to Study Its Regulatory Role in Non-Small Cell Lung Cancer

Abstract

KRAS is the particularly prevalent oncogene in non-small cell lung cancer (NSCLC).Single nucleotide polymorphism (SNP) can add, take away, or alter protein coding sites and can lead to complicated illnesses. KRAS stands for Kirsten Ras that provides instructions for the production of K-Ras protein which plays an important part in RAS/MAPK signaling pathway. This study entailed an in-depth investigation on human KRAS nsSNPs suspected of illness. From NCBI's dbSNP, the nsSNPs were obtained, and numerous sequence and structure based analysis were carried out to screen out the most affecting SNPs. Tools like Poly-Phen-2, Meta-SNP, PMut,SNAP2, PhD-SNP, SNP&GO, I-Mutant2.0, Align GVGD were used for the analysis. Then phenotypic consequences were analyzed for these SNPs.