RNA-Seq Analysis of HeLa cells Over-expressing Histone Methyltransferase KMT2B

Abstract

Nearly all fields of biotechnology have been affected by Next-generation sequencing (NGS), which has emerged as a major method in the field of genomics. Thanks to its exceptional throughput, flexibility, and fast data collection, it enables researchers and clinicians to analyze biological systems at a level and precision that was previously unimaginable. By using RNA sequencing analysis, NGS has contributed to identifying Differentially expressed genes (DEGs) in over-expressing KMT2B heLa cells and control heLa cells. We found 72,157 total DEG’s, out of them 4,005 are significant DEG’s. From total significant DEG’s 1,747 are upregulated genes and 2,257 are downregulated DEG’s.