Cloning and Expression of Arginase in E. coli

Abstract

As per the statistics published in 2022 by the American Cancer Society, cancer is the second leading cause of death worldwide and although there are a number of treatment methods developed, they have several side effects like heart problems, dysphagia, chronic bladder spams associated with it. Also, these techniques to treat cancers are very expensive, so with the increasing research in the field of cancer a new treatment method was identified that is depletion of semi essential amino acids that are needed by cancerous cells. There were several cancer cells that were identified to have an important feature of arginine auxotrophy, for which those cancer cell lines were dependent on the extracellular nutritional pool to fulfil their nutrient demand and with the increasing research it was found that enzymatic degradation of arginine is one of the most efficient approaches to deprive arginine.