Production and Characterization of Esterase Producing Bacterial Strain

Abstract

Esterases (EC 3.1.1.x) are a family of hydrolases that catalyze the synthesis and breakdown of ester bonds. They're found in a wide range of sources including animals, plants, and microbes, and many of them have a wide range of substrate tolerance, implying that they developed to have access to carbon sources or to participate in the catabolic process. Esterases are one of the most commonly utilised biocatalysts since they don't require cofactors, are usually quite stable, and can even function in organic solvents. Esterase activity is often checked using either chromophoric compounds (e.g. p-nitro phenyl esters) or tributyrin-supplemented agar plates. (Bornscheuer ,2002 ) and the activity can be measured in terms of amount of enzyme that releases 1 μmol of p-nitro phenol from a p-nitrophenyl ester per minute (Sood et al., 2016).